Identification of human short introns
نویسندگان
چکیده
منابع مشابه
Identification of human short introns
Canonical pre-mRNA splicing requires snRNPs and associated splicing factors to excise conserved intronic sequences, with a minimum intron length required for efficient splicing. Non-canonical splicing-intron excision without the spliceosome-has been documented; most notably, some tRNAs and the XBP1 mRNA contain short introns that are not removed by the spliceosome. There have been some efforts ...
متن کاملIdentification and Validation of Evolutionarily Conserved Unusually Short Pre-mRNA Introns in the Human Genome
According to the length distribution of human introns, there is a large population of short introns with a threshold of 65 nucleotides (nt) and a peak at 85 nt. Using human genome and transcriptome databases, we investigated the introns shorter than 66 nt, termed ultra-short introns, the identities of which are scarcely known. Here, we provide for the first time a list of bona fide human ultra-...
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analyzing the gene of interest has several advantages over subcloning analysis. This method does not require any information about physical maps of the insert DNA. Positions of transposon insertion are presented as a distance from one end of the genomic DNA. Even if the restriction map is known, this technique should be preferable because of its simplicity. Using this protocol, it takes only on...
متن کاملIdentification of a family of group II introns encoding LAGLIDADG ORFs typical of group I introns.
Group I and group II introns are unrelated classes of introns that each encode proteins that facilitate intron splicing and intron mobility. Here we describe a new subfamily of nine introns in fungi that are group II introns but encode LAGLIDADG ORFs typical of group I introns. The introns have fairly standard group IIB1 RNA structures and are inserted into three different sites in SSU and LSU ...
متن کاملComputational identification of functional introns: high positional conservation of introns that harbor RNA genes
An appreciable fraction of introns is thought to have some function, but there is no obvious way to predict which specific intron is likely to be functional. We hypothesize that functional introns experience a different selection regime than non-functional ones and will therefore show distinct evolutionary histories. In particular, we expect functional introns to be more resistant to loss, and ...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2017
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0175393